BIOMARKER ACTIVITIES
TARGETED LIPIDOMIC: QUANTIFICATION OF NON-ENZYMATIC OXYLIPINS
Since 2014, our team has specialized in the LC-MS quantification of oxidized metabolites in various human, animal and plant matrices using a targeted lipidomics approach. The synthesis of oxylipins plays a key role in targeted lipidomics quantification, as this approach requires the use of pure compounds as standards in order to detect and quantify them in complex biological matrices containing thousands of different oxylipins.
Our team has therefore developed a separation method enabling the separation and quantification of over 54 metabolites. Different sample processing protocols have also been developed, and these studies have enabled us providing an analytical platform to quantify non-enzymatic oxylipins.
Our team has been working on various matrices: human (blood, plasma, urine, sperm, CSF...), animal (zebrafish, trout, ...) and terrestrial plants and derivatives (melon leaves, acacia, coffee, cocoa, date, pumpkin...), and marine plants and derivatives (macroalgae, microalgae, fish-based oil). These studies have led to the publication of more than 18 articles (see publications).
OUR WORK ON MARINE MATRICES
OUR COLLABORATIONS
Our team is working with scientists all over the World. Our goal is to discover if non-enzymatic metabolites of PUFA are potent and specific biomarkers of pathologies linked to oxidative stress. Thus, several collaborations are developped with analytic chemists and clinicians.